COMPOUNDS AND REAGENT COMPOSITIONS AND THEIR USE IN THE QUANTITATIVE DETERMINATION OF RETICULOCYTES IN WHOLE BLOOD

摘要

Quaternized derivatives of acridine orange and reagents incorporating such derivatives and their use in quantitatively determining reticulocyte levels in whole blood specimen by fluorescence flow cytometry techniques are disclosed. The quarternized derivatives of acridine orange are of the general formula: wherein Y is Br? or I? wherein X may be R1 and/or R2 substituted benzyl group R1 is hydrogen or fluorine, and R2 is fluorine, trifluoromethyl or hydrogen, or X may be hydroxyl ethylene.