Polymers derived from polystyrenes and cross-linked dextranes, their preparation and their use in the analysis and the purification of biologic molecules.

摘要

These resins derived from a crosslinked styrene (co)-polymer, or from a crosslinked dextran, comprise a (co)-polymer chain which is substituted with one or more groups, which may be identical or different, belonging to the following categories: -Z-A1; -Z-A2; -Z-A1-Z'-A2; -Z-A1-A3-A2; -Z-A1-A4, where Z=spacer chain, Z'=linking chain, A1=phosphate residue, A2=residue of purine or pyrimidine base, A3=sugar residue and A4=residue of a molecule participating in the polar structure of various phospholipids. These resins are applicable for the analysis and purification of molecules of biological origin, in particular as a stationary phase in ion-exchange and affinity chromatography, especially for carrying out the fractionation of protein mixtures. Their interaction with proteins, in particular the anti-DNA and anti-phospholipid antibodies present in the serum of patients suffering from SLE, makes them applicable to operations for the selective purification of the different types of antibodies developed by lupus patients.