Process for the construction of an animal cell line for the production of human beta-interferon.

摘要

A process for the production of beta-IFN in an animal host cell is described. The beta-IFN isolated in high yield from the cell supernatant has a specific activity of about 3.8 x 10<8> IU/mg of protein. Biological and immunological tests show that the isolated beta-IFN is to the greatest extent identical to natural beta-IFN. The beta-IFN produced by the process described is glycosylated to 95%, the glycosylation agreeing to a large extent in structure and sequence with that of natural beta-IFN, but without being identical to it.