METHOD FOR THE PURIFICATION OF INSULIN

摘要

A method for the purification of insulins and insulin derivatives by chromatography in aqueous buffered solvents which contain water-miscible organic solvents on lipophilically modified silica gel is described and entails the aqueous buffered solvents containing zwitterions or the pH of the solvent mixture being in the vicinity of the isoelectric point of the insulin or insulin derivative to be purified, and zwitterions being present.