Hammerhead ribozymes with enhanced stability provided by an additional 3' hairpin sequence
摘要
A DNA fragment is disclosed which has connected to the downstream side of a first DNA sequence containing a region coding for the first ribozyme RNA a second DNA sequence containing a region coding for second ribozyme RNA capable of cleaving by self-processing the 3'-terminus site of the first ribozyme RNA subjected to transcription. A ribozyme having the 3'-terminus site thereof self-processed is obtained by effecting transcription of a RNA using as a template a recombinant vector obtained by recombination by the use of the DNA fragment.
申请公布号
US5436330(A)
申请公布日期
1995.07.25
申请号
US19930111444
申请日期
1993.08.24
申请人
AGENCY OF INDUSTRIAL SCIENCE & TECHNOLOGY;MINISTRY OF INTERNATIONAL TRADE & INDUSTRY