发明名称 |
PURIFICATION OF RETROVIRAL VECTORS |
摘要 |
A method of purifying retroviral vector particles which comprises culturing retroviral vector producer cells which are capable of generating retroviral vector particles, and obtaining a supernatant containing retroviral vector particles from the culture of retroviral vector producer cells. The supernatant is clarified, and then concentrated. The supernatant then is contacted with a precipitation agent, and the supernatant and precipitation agent are centrifuged. A precipitate is recovered, resuspended, and subjected to a high-speed centrifugation. Retroviral particles then are recovered. The above method provides for increased recovery of retroviral vector particles which may be employed in gene therapy.
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申请公布号 |
CA2181066(A1) |
申请公布日期 |
1995.07.20 |
申请号 |
CA19952181066 |
申请日期 |
1995.01.10 |
申请人 |
GENETIC THERAPY, INC. |
发明人 |
KOTANI, HITOSHI;NEWTON, PERRY III;ZHANG, SHUYUAN |
分类号 |
C12N15/09;C12N7/02;C12N15/00;C12N15/867;C12R1/92;(IPC1-7):C12N7/04;C12N15/86 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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