PRODUCTION OF L-ISOLEUCINE BY MEANS OF RECOMBINANT MICRO-ORGANISMS WITH DEREGULATED THREONINE DEHYDRATASE

摘要

The invention relates to processes for the microbial production of L-isoleucine. To this end, in a gene in vitro of a threonine dehydratase, one or more bases in the gene region coding the enzyme's allosteric domains is/are exchanged in such a way that at least one amino acid in the amino acid sequence of the allosteric domains of the enzyme is replaced by another so that the enzyme is no longer inhibited by L-isoleucine feedback. Furthermore, concrete amino acid exchanges in the amino acid sequence of the enzyme are effected in a gene in vitro of a threonine dehydratase of corynebacterium glutamicum by base exchange both outside and inside and outside the gene region coding the allosteric domains of the enzyme so that, after the transformation of such mutated threonine de hydratase genes into a threonine or L-isoleucine-producing host cell, the latter repeatedly forms L-isoleucine.