发明名称 |
Thrombolytic agent and process of preparation thereof |
摘要 |
A process for the preparation of thrombinase. This process includes the steps of subjecting a cell free culture filtrate from a culture broth of the fermentation of Bacillus sphaericus serotype H5a5b to ultra filtration to obtain a concentrated retentate. Substantially all the protein is then salted out and the salted out protein is converted into an aqueous solution. This solution is subjected to decolorization, dialysis and lyophilization to obtain a crude powder. A solution is prepared from the crude powder and subjected to chromatography treatment to obtain a purified material which is then lyophilized.
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申请公布号 |
US5434059(A) |
申请公布日期 |
1995.07.18 |
申请号 |
US19930119009 |
申请日期 |
1993.09.09 |
申请人 |
BALARAMAN, KOTHANDAPANI;KUPPUSAMY, MUSAVAN |
发明人 |
BALARAMAN, KOTHANDAPANI;KUPPUSAMY, MUSAVAN |
分类号 |
A61K38/00;C12N9/54;C12P21/04;(IPC1-7):C12P21/04;C12N9/48 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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