Method of proteolytically cleaving prothrombin to produce thrombin

摘要

Prothrombin is cleaved by proteases in the presence of a detergent or certain chaotropic substances to produce thrombin. Under these conditions, controlled and restricted proteolysis occurs such that significant activation without digestion occurs. The chaotropic substances may be urea, guanidinium hydrochloride or a thiocyanate salt. Reversible immobilization of prothrombin on a solid support prior to activation improves the selectivity of the proteolytic activation.