发明名称 |
Simultaneous calibration heterogeneous immunoassay |
摘要 |
An assay method and compositions are provided for determining the presence of an analyte in a sample. The analyte is a member of an immunological pair (mip) comprising ligand and receptor. By providing a first measurement surface capable of specifically binding a labelled reagent in an amount depending upon the presence of analyte in the sample and a second calibration surface capable of binding a second labeled reagent in a manner unaffected by the presence of analyte in the sample, calibration of individual tests can be accomplished simultaneously with the performance of the test itself. A signal producing system includes an enzyme, a catalyst usually bonded to a mip which defines the first labeled reagent for binding to the measurement surface and the same catalyst conjugated to a ligand capable of binding to the calibration surface. Preferably, both labeled reagents have the same composition and the calibration surface includes anti-(first catalyst).
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申请公布号 |
US5432057(A) |
申请公布日期 |
1995.07.11 |
申请号 |
US19920937252 |
申请日期 |
1992.08.28 |
申请人 |
SYVA COMPANY |
发明人 |
LITMAN, DAVID J.;ULLMAN, EDWIN F. |
分类号 |
G01N33/52;G01N33/542;G01N33/543;G01N33/58;(IPC1-7):G01N33/543 |
主分类号 |
G01N33/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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