| 摘要 |
The present invention provides a method, device and test kit for determining the concentration of analytes in liquid samples in which the amount of binding agent having binding sites specific for a given analyte in the liquid sample is immobilised in a test zone on a solid support, the binding agent being divided into an array of spatially separated locations in the test zone. The concentration of the analyte is obtained by back-titrating the occupied binding agent with a developing agent having a marker and integrating the signal from each location in the array. The assay has the advantage of avoiding the increasing statistical errors as the area of a prior art microspot is reduced, while optimising signal-to-noise and the kinetics of the reaction between analyte and binding agent.
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