Affinity sepn. method using dynamic filtration device

摘要

The present invention provides an affinity separation method comprising (a) introducing affinity particles and a fluid containing a target compound to be isolated which is capable of adsorbing onto the affinity particles into a dynamic filtration apparatus comprising motive means, a filtration medium having upstream and downstream sides, an inlet in fluid communication with the upstream side of the filtration medium, and a filtrate outlet in fluid communication with the downstream side of the filtration medium, wherein the fluid is capable of passing through the filtration medium and the affinity particles are substantially incapable of passing through the filtration medium, (b) operating the motive means of the dynamic filtration apparatus with the filtrate outlet being in a closed position such that the fluid cannot pass through the filtration medium under conditions sufficient to allow for the target compound to adsorb onto the affinity particles, and (c) separating the affinity particles from the fluid by opening the filtrate outlet so as to allow the fluid to pass through the filtration medium of the dynamic filtration apparatus. The affinity particles can then be washed, and the target compound eluted from the affinity particles. Also disclosed are (1) a method of sizing affinity particles comprising: (a) filtration apparatus comprising motive means, a filtration medium having upstream and downstream sides, an inlet in fluid communication with the upstream side of said filtration medium, and a filtrate outlet in fluid communication with the downstream side of said filtration medium, (b) filtration apparatus with said filtrate outlet being in an open position such that any of said substrate particles having a diameter significantly smaller than the pore size of said filtration medium pass through said filtration medium, and (c) pass through said filtration medium from said dynamic filtration apparatus, and (2) An affinity particle comprising a substrate and a ligand attached thereto, said substrate comprising a core material and a nonporous, hydrophilic polymer coating thereon.