METHOD OF ASSAY USING TWO DISTINGUISHABLE TYPES OF PARTICLES

摘要

A binary assay method capable of providing wide dynamic range and a high degree of precision in which analyte and labelled ligand are reacted with two independently determinable forms of solid-supported binding partners having affinity for the analyte and labelled ligand respectively, the analyte concentration being determined from signals deriving from the resulting two forms by reference to a double standard calibration curve. A kit for use in the method is also disclosed.