Sequencing of oligosaccharides

摘要

PCT No. PCT/GB92/00831 Sec. 371 Date Nov. 3, 1993 Sec. 102(e) Date Nov. 3, 1993 PCT Filed May 7, 1992 PCT Pub. No. WO92/19766 PCT Pub. Date Nov. 12, 1992.The present invention relates to the analysis of unknown oligosaccharides and more particularly the form of analysis in which an unknown oligosaccharide is sequentially brought together with different agents which cause monosaccharides to be cleaved from (or chemically bound to) the oligosaccharide. If cleavage occurs the monosaccharide will subsequentially be detected in the products of the experiment thus confirming that the monosaccharide was attached to the unknown oligosaccharide and providing a means of determining the structure of the unknown oligosaccharide. The invention proposes an analytical technique for determining the best agent to be used in performing such an experiment so that a maximum amount of information is obtained from the experiment.