| 摘要 |
PURPOSE:To obtain the subject signal peptide, having a specific amino acid sequence and capable of selectively providing only a thrombomodulin, manifesting anticoagulant actions, useful as an active ingredient of an anticoagulant and having an N-terminal amino acid sequence essential thereto. CONSTITUTION:This signal peptide for expressing thrombomodulins has an amino acid sequence expressed by the formula. The thrombomodulins having an N-terminal amino acid sequence essential thereto are obtained by joining a base sequence capable of coding the amino acid sequence of the signal peptide, synthesized according to a solid-phase synthetic method, etc., and used for expressing the thrombomodulins, a base sequence composed of an initiation codon, a liposome-binding site and a promoter to the upstream side of a base sequence capable of coding an amino acid sequence of the thrombomodulins, joining a termination codon and a base sequence capable of acting as a polyA addition signal to the downstream side of the base sequence capable of coding the thrombomodulins, synthesizing a recombinant vector, transducing the resultant recombinant vector into a host cell, transforming the host cell, then culturing the transformant cell and collecting the obtained product from the culture. |
