发明名称 |
METHOD FOR SIMULTANEOUS AMPLIFICATION OF NUCLEIC ACID |
摘要 |
Nucleic acids can be amplified and detected using a very rapid polymerase chain reaction procedure in which two different nucleic acid sequences are present. This method allows one to preferentially modulate (for example, suppress) the degree of amplification of one or more nucleic acid sequences relative to other nucleic acid sequences. This modulation is achieved by exploiting differences in the relative primer melt temperatures, or by using certain ratios of primers. Each PCR cycle is very fast, that is less than 90 seconds. This method is particularly useful for amplification and detection of DNA associated with infectious agents that may be present in a specimen in very small quantities compared to other nontargeted nucleic acids. |
申请公布号 |
JPH07163370(A) |
申请公布日期 |
1995.06.27 |
申请号 |
JP19940155747 |
申请日期 |
1994.07.07 |
申请人 |
EASTMAN KODAK CO |
发明人 |
JIYON DABURIYU BATSUKASU;UIRIAMU HARORUDO DONITSUSHIYU;JIYON BURUUSU FUINDOREI;JIYON UIRIAMU EICHI SAZAARANDO;MAAREN EMU KINGU |
分类号 |
C12N15/09;C12Q1/68;C12Q1/70 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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