ORIGIN OF REPLICATION COMPLEX GENES, PROTEINS AND METHODS

摘要

Origin of DNA Replication Complex (ORC) genes, recombinant ORC peptides and methods of identifying DNA binding proteins and using the subject compositions are provided. Vectors and cells comprising such ORC genes find use in the production of recombinant ORC peptides. The subject ORC peptides find particular use in screening for ORC selective agents useful in the diagnosis, prognosis or treatment of disease, particularly fungal infections and neoproliferative disease. Disclosed methods for identifying a gene encoding a protein which directly or indirectly associates with a selected DNA sequence involve: transforming an expression library of hybrid proteins into a reporter strain, wherein the library comprises protein-coding sequences fused to a constitutively expressed transcription activation domain and the reporter strain comprises a reporter gene with at least one copy of a selected DNA sequence in its promoter region. Clones expressing the transcription or translation product of the reporter gene are detected and recovered.