发明名称 |
METHOD OF HIGHLY PURIFYING HUMAN SERUM ALBUMIN. |
摘要 |
<p>A method of highly purifying human serum albumin (HSA) which comprises bringing a fraction containing HSA produced by gene manipulation into contact with a chelate chromatography carrier having copper ions combined therewith and eluting the HSA adsorbed on the carrier by using a buffer having a pH of 5 to 7 and containing ammonium chloride as an antagonist. This method can provide a highly pure HSA free from yeast-origin components which have been dificult to remove satisfactorily by the conventional method of purifying HSA produced by gene manipulation.</p> |
申请公布号 |
EP0656419(A1) |
申请公布日期 |
1995.06.07 |
申请号 |
EP19930916225 |
申请日期 |
1993.07.26 |
申请人 |
MITSUBISHI PHARMA CORPORATION |
发明人 |
FULUHATA, NAOTO;SUMI, AKINORI;OHMURA, TAKAO |
分类号 |
C07K14/76;C07K1/16;C07K1/20;C07K14/765;C12N15/09;C12N15/81;C12P21/00;C12P21/02;C12R1/84;(IPC1-7):C12P21/02 |
主分类号 |
C07K14/76 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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