发明名称 |
Method of site specific nucleic acid cleavage |
摘要 |
A method of cleaving a target nucleic acid molecule is disclosed. A cleavage structure is formed that comprises the target nucleic acid and a pilot nucleic acid. A first region of the target nucleic acid is annealed to the pilot nucleic acid to form a duplex structure. A second region of the target nucleic acid contiguous to the duplex is not annealed to the pilot nucleic acid, thus forming a junction site between the duplex region and the non-annealed region. The cleavage structure is exposed to a cleavage agent capable of preferentially cleaving the cleavage structure at a target site in a manner independent of the sequence of the cleavage structure. The cleavage structure and the cleavage agent are incubated under conditions wherein cleavage can occur.
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申请公布号 |
US5422253(A) |
申请公布日期 |
1995.06.06 |
申请号 |
US19920986330 |
申请日期 |
1992.12.07 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
DAHLBERG, JAMES E.;LYAMICHEV, VICTOR I.;BROW, MARY A. D. |
分类号 |
C07H21/00;C07K14/47;C12N9/12;C12N15/09;C12N15/10;C12N15/113;C12Q1/68;C12Q1/70;G01N33/53;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C07H21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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