Synthesis of Lex; dimeric Lex (difucosyl Y2; III3FucV3FucnLc6Cer); sialylated forms thereof; and analogues thereof

摘要

A process for preparing difucosyl Y2 antigen (dimeric Lex) , said process comprising: (1) preparing a lactonorhexaosylceramide backbone or a lactonorhexaosylsaccharide backbone linked to a carrier molecule; and (2) enzymatically fucosylating said backbone at the III3 and V3 positions through an alpha 1->3 linkage. A process for preparing Ley antigen analogues, said process comprising: (1) preparing a lactonorhexaosyl-ceramide backbone or a lactonorhexaosylsaccharide backbone linked to a carrier molecule; and (2) enzymatically fucosylating said backbone at the terminal beta -Gal through an alpha 1->2 linkage; and (3) enzymatically fucosylating said backbone at one or more positions through an alpha 1->3 linkage, provided that steps (2) and (3) can be conducted simultaneously or in any order. A process for preparing a fucosylated lactonorhexaosylceramide, lactonorhexaosylsaccharide linked to a carrier molecule or higher analogues thereof, said process comprising: (1) preparing a lactonorhexaosylceramide backbone, a lactonorhexaosylsaccharide backbone linked to a carrier molecule or backbones of higher analogues thereof; and (2) enzymatically fucosylating one or more residues of said backbone. A process for preparing sialyl Lex and sialyl dimeric Lex.