发明名称 |
VECTOR SYSTEMS FOR THE GENERATION OF ADENO-ASSOCIATED VIRUS PARTICLES |
摘要 |
A vector system comprising a first vector, which is an adeno-associated viral vector, and which includes an adeno-associated virus 5'ITR, an adeno-associated virus 3'ITR, and at least one heterologous DNA sequence. The vector system also includes a second vector which includes an inducible origin of replication, such as an SV40 origin of replication, which is capable of being induced or activated by an agent, such as the SV40 T-antigen. The second vector also includes DNA sequences encoding the adeno-associated virus rep and cap proteins. When induced by an agent, the second vector may replicate to a high copy number, and thereby increased numbers of infectious adeno-associated viral particles may be generated.
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申请公布号 |
WO9514771(A1) |
申请公布日期 |
1995.06.01 |
申请号 |
WO1994US13516 |
申请日期 |
1994.11.21 |
申请人 |
GOVERNMENT OF THE UNITED STATES, REPRESENTED BY TH;GENETIC THERAPY, INC. |
发明人 |
KOTIN, ROBERT;CHIORINI, JOHN, A.;SAFER, BRIAN;URCELAY, ELENA |
分类号 |
C12N15/09;C12N5/10;C12N15/864;(IPC1-7):C12N15/00;C12N15/34;C12N15/37;C12N15/86 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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