| 摘要 |
PURPOSE:To provide an enzyme from periodontic bacteria enabling sure determination of the progress and activity of periodontosis without using the experience of skilled person and expectable as a preventing and treating agent for periodontosis in addition to the use as an analytic reagent. CONSTITUTION:This invention relates to an enzyme originated from Porphyromonas gingivalis and having the following enzymological properties, a determination method for the enzyme and an antibody against the enzyme. (1) Action, decomposing periodontal tissue and inhibiting inflammatory cells; (2) substrate specificity, exhibiting high decomposition activity against various proteins such as collagen and immunoglobulin and strong specific decomposition activity against synthetic fluorescent substrate; (3) optimum pH of 7-8 and stable pH of 4-9; (4) suitable working temperature range, from room temperature to 37 deg.C; (5) molecular weight, about 50kDa by gelfiltration and about 44kDa by SDS get electrophoresis; (6) isoelectric point, pH 5 to 5.5. |
