ANTIHUMAN TYROSINASE MONOCLONAL ANTIBODY

摘要

A human tyrosinase is detected by using a monoclonal antibody prepared by immunizing a mammal by using as an antigen a peptide having that region of the amino acid sequence of a human tyrosinase which is low in the homology with the amino acid sequence of a tyrosinase-associated protein, preparing a hybridoma by fusing the splenocytes of the mammal and cultured cells, culturing the hybridoma, and harvesting a monoclonal antibody that combines with human and mouse tyrosinases but does not combine with the tyrosinase-associated protein from the medium.