发明名称 |
Chromosome characterization using single fluorescent dye |
摘要 |
Chromosomes are characterized by fluorescent emissions from a single fluorescent dye that is excited over two different wavelengths. A mixture containing chromosomes is stained with a single dye selected from the group consisting of TOTO and YOYO and the stained chromosomes are placed in a flow cytometer. The fluorescent dye is excited sequentially by a first light having a wavelength in the ultraviolet range to excite the TOTO or YOYO to fluoresce at a first intensity and by a second light having a wavelength effective to excite the TOTO or YOYO dye to fluoresce at a second intensity. Specific chromosomes may be identified and sorted by intensity relationships between the first and second fluorescence emissions.
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申请公布号 |
US5418169(A) |
申请公布日期 |
1995.05.23 |
申请号 |
US19940218090 |
申请日期 |
1994.03.25 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, OFFICE OF TECHNOLOGY TRANSFER |
发明人 |
CRISSMAN, HARRY A.;HIRONS, GREGORY T. |
分类号 |
G01N21/64;(IPC1-7):G01N21/64 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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