| 摘要 |
The present invention provides a viable zygote in which chromosomal DNA from one of the parental gametes has been substantially degraded. The invention also provides: organisms, particularly plants, resulting from cell division of the zygote; and a method of producing a viable zygote having an altered chromosome complement when compared with the wild type - comprising fertilizing a maternal gamete with a paternal gamete - characterized in that in the zygote, chromosomal DNA from one of the gametes is substantially degraded. The paternal gamete is preferably treated with a DNA intercalating agent in the dark, fertilization of the maternal gamete takes place in the dark, and the zygote is subjected to visible and/or ultra-violet light before or after its first mitotic division. |
