A method for producing in culture phenylalanine dehydrogenase at yields of 50+ units of enzyme per litre of culture, one unit measured as the amount required to convert one micro-mole of phenylalanine to phenyl pyrovic acid at 25 DEG C. and pH 10.8, in a growth medium comprising a nitrogen source, a buffer, phenylalanine or phenylpyruvic acid or a salt thereof at 20 mM and optionally a vitamin solution. Using phenylalanine at about 100 mM gives yields of phenylalanine dehydrogenase enzyme of 350+ units per liter of culture.
申请公布号
WO9511297(A3)
申请公布日期
1995.05.18
申请号
WO1994GB02298
申请日期
1994.10.20
申请人
MICROBIOLOGICAL RESEARCH AUTHORITY;HAMMOND, PETER, MICHAEL;BREARLEY, GRAHAM, MARK;PRICE, CHRISTOPHER, PHILIP
发明人
HAMMOND, PETER, MICHAEL;BREARLEY, GRAHAM, MARK;PRICE, CHRISTOPHER, PHILIP