METHOD FOR PRODUCING TAXOL AND ITS DERIVATIVES FROM EMBRYO CULTURES OF TAXUS SPECIES

摘要

The taxol production method using somatic cell culture consists of (1) culturing seeds of Taxus cuspidata to form callus, (2) isolating somatic cells from callus in hormone free medium, (3) culturing somatic cells in nicotinic acid 1-2 ppm containing medium, (4) adding phenylalanine, gibberelline, pestalotiopsis sp. which is contaminated on Taxus tree to produce taxol in 4.1-6.3 mg/l.