发明名称 |
DNA encoding ACV synthetase. |
摘要 |
DNA encoding the gene for the synthetase enzyme capable of generating delta (L- alpha -aminoadipyl)-L- cysteinyl-D-valine (ACV) from its constituent amino acids was obtained from several penicillin and cephalosporin producing organisms, e.g. Penicillium chrysogenum and a Flavobacterium species. The DNA was used to prepare recombinant vectors comprising the ACV synthetase gene and hosts transformed with such vectors. The ACV synthetase gene can form part of a gene cluster comprising other genes involved in beta -lactam biosynthesis and the production of penicillin by expression of the entire biosynthetic gene cluster for the synthesis of penicillin from primary amino acids is described. Suitable hosts in which expression can take place include heterologous hosts which are naturally non-producers of penicillin. |
申请公布号 |
EP0320272(B1) |
申请公布日期 |
1995.05.17 |
申请号 |
EP19880311655 |
申请日期 |
1988.12.09 |
申请人 |
BEECHAM GROUP PLC |
发明人 |
BURNHAM, MARTIN KARL RUSSELL;EARL, ALISON JANE;BULL, JOHN HENRY UNIVERSITY OF BRISTOL;SMITH, DAVID JOHN UNIVERSITY OF BRISTOL;TURNER, GEOFFREY UNIVERSITY OF BRISTOL |
分类号 |
C12N15/09;C12N1/15;C12N9/00;C12P37/00;C12R1/82 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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