| 摘要 |
This invention relates to a method of producing a recombinant eukaryotic heterodimeric enzyme using a prokaryotic host. The method involves constructing a first DNA vector containing DNA encoding one of the subunits of the dimeric emzyme and then constructing a second DNA vector containing DNA encoding the second subunit of the enzyme. Once the DNA vectors are constructed, they are used to transform a prokaryotic host. The transformed prokaryotic host cell is then cultured under conditions appropriate for the expression of the dimeric enzyme. For example, using the method of the present invention, the heterodimeric isoform of creating kinase CKMB can be produced. This invention further relates to a method of producing a recombinant human dimeric enzyme in an active form using a prokaryotic host, the recombinant enzyme products produced using the method of the present invention, and a transformed prokaryotic host constructed by the method of the present invention.
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