Method for making conjugate moieties capable of chelating paramagnetic metals and designed for coupling with a factor responsive to specific cellular marker sites

摘要

PCT No. PCT/EP92/01560 Sec. 371 Date Feb. 18, 1993 Sec. 102(e) Date Feb. 18, 1993 PCT Filed Jul. 19, 1992 PCT Pub. No. WO93/01837 PCT Pub. Date Feb. 4, 1993.In this method, one attaches one or more chelatant molecules to a mono- or polyamino intermediate compound which is temporarily immobilized on a solid phase by a splittable bond. Thereafter, said bond is split to release the desired conjugate moiety, whereby a reactive site is generated at the splitting site. The conjugate can be coupled to a protein homing factor using said reactive site; the latter being single per chelatant molecule, undesirable cross-linking during conjugation is substantially avoided.