EXPRESSION SYSTEM FOR EUKARYOTIC CELL LINES

摘要

It is disclosed a process for the preparation of recombinant human G-CSF for which is used a vector system which comprises at least one of the following elements: (1) a CMV promoter upstream of a DNA sequence encoding the desired protein, (2) a sequence for processing of RNA transcripts, and (3) a selection and amplification marker sequence; it is furtherly disclosed the use of such recombinant human G-CSF for the preparation of pharmaceutical compositions for promoting hematopoiesis, for the augmentation of the defence mechanism against infection and for the stimulation and hyperproduction of functionally primed effector cells against malignant diseases.