| 摘要 |
<p>A cultivation process including steps (i) recombinant E. coli cells which include a promoter coupled to a gene which encodes a recombinant protein wherein said promoter is normally inducible by IPTG in a cultivation vessel during a growth phase at temperatures from room temperature to 32 °C wherein the recombinant protein is (a) expressed at a low level and (b) is substantially non-toxic to E. coli whereby inclusion bodies are not formed in step (ii) hereinafter; and (ii) initiating an induction phase by application of heat to the cultivation vessel in step (i) so as to enhance the level of production of the recombinant protein.</p> |
