METHODS OF SCREENING FOR BETA-AMYLOID PEPTIDE PRODUCTION INHIBITORS

摘要

Processing of β-amyloid precursor protein (βAPP) is monitored by detecting the secretion of a soluble amino-terminal fragment or βAPP (ATF-βAPP) resulting from cleavage of βAPP at the amino-terminus of β-amyloid peptide. Secretion of ATF-βAPP in animal models may be monitored to identify inhibitors of β-amyloid production. The ATF-βAPP may be detected using antibodies and other specific binding substances which recognize a carboxy terminal residue on the fragment. Animals expressing the Swedish mutation of βAPP are described which produce abundant amounts of ATF-βAPP.