METHODS OF SCREENING FOR BETA-AMYLOID PEPTIDE PRODUCTION INHIBITORS

摘要

Processing of beta -amyloid precursor protein ( beta APP) is monitored by detecting the secretion of a soluble amino-terminal fragment or beta APP (ATF- beta APP) resulting from cleavage of beta APP at the amino-terminus of beta -amyloid peptide. Secretion of ATF- beta APP in animal models may be monitored to identify inhibitors of beta -amyloid production. The ATF- beta APP may be detected using antibodies and other specific binding substances which recognize a carboxy terminal residue on the fragment. Animals expressing the Swedish mutation of beta APP are described which produce abundant amounts of ATF- beta APP.