| 摘要 |
<p>A method is disclosed for automated multiplex sequencing of DNA with an integrated system. This system comprises a hybridization chamber for a membrane containing size-fractionated multiplex sequencing reaction products, apparatus for fluid delivery to the chamber, imaging apparatus for image recording of fluorescence emanating from the membrane, and programmable controller apparatus for controlling operation of the system. The multiplex reaction products are hybridized with a probe, then an enzyme is bound to the probe, and a fluorogenic substrate is introduced into the chamber by the fluid delivery apparatus. The enzyme converts the fluorogenic substrate into a fluorescent product which, when illuminated in the chamber, produces a fluorescence pattern of hybridization. The pattern of hybridization is imaged by a CCD camera to obtain a series of digital signals, which are converted by the controller apparatus into a nucleotide sequence. The apparatus can also be used for colony and plaque hybridization, and Southern, Northern, and Western blots.</p> |
