发明名称 |
METHOD OF FORMING N-PROTECTED AMINO ACID THIOHYDANTOINS. |
摘要 |
<p>A method of forming a thiohydantoin from an N-protected amino acid. The method employs a uronium or phosphonium compound to activate the terminal carboxyl group of the amino acid and a thiocyanate reagent to cyclize the activated amino acid to the thio-hydantoin. The thiohydantoin can be cleaved from its N-protecting group, for use in C-terminal peptide sequencing. Particularly preferred uronium compounds include salts of 2-chlorouronium. Preferred thiocyanate reagents include trimethylsilyl isothiocyanate and crown ether adducts of metallothiocyanates, such as the 18-crown-6 adduct of KSCN.</p> |
申请公布号 |
EP0650478(A1) |
申请公布日期 |
1995.05.03 |
申请号 |
EP19930917177 |
申请日期 |
1993.07.14 |
申请人 |
APPLIED BIOSYSTEMS, INC. |
发明人 |
BOYD, VICTORIA, L.;BOZZINI, MERILISA;GUGA, PIOTR, J.;ZON, GEROLD |
分类号 |
C07D233/86;C07K1/12;G01N33/68;(IPC1-7):C07D233/86;C07K1/08 |
主分类号 |
C07D233/86 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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