| 摘要 |
PCT No. PCT/JP91/00923 Sec. 371 Date May 5, 1992 Sec. 102(e) Date May 5, 1992 PCT Filed Jul. 10, 1991 PCT Pub. No. WO92/01043 PCT Pub. Date Jan. 23, 1992.A thymidine kinase-lacking hybrid cell having an Ig-producing ability in which chicken B lymphoblast with immunized chicken spleen cell, a method of obtaining the above hybrid cell which comprises mutating chicken B lymphoblast cell, isolating a thymidine kinase-lacking cell using a culture medium containing a thymidine analog, fusing it with immunized chicken spleen cell, mutating the hybrid cell, and isolating a thymidine kinase-lacking hybrid cell using a culture medium containing a thymidine analog, a hybrid cell having an IgG-producing ability in which a chicken spleen cell immunized with an antigen is further fused with the above hybrid cell, a method of producing a chicken IgG which comprises culturing the above hybrid cell having an IgG-producing ability in a culture medium. The antibody-producing cell of the invention can produce chicken IgG stably for a long period, and thereby it opens the way for the massproduction of chicken IgG.
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