EXPRESSION OF RECOMBINANT BILE SALT ACTIVATED LIPASE IN HIGH YIELD

摘要

PURPOSE:To enable the expression of a recombinant bile salt activated lipase useful for the treatment of pancreatic insufficiency, etc., in high yield by culturing a specific transformant under specific condition. CONSTITUTION:A gene coding a secretory signal is linked to a defective gene fragment obtained by deleting a part or total of the gene coding an amino acid sequence consisting of 16 repeated structures containing a peptide composed of 11 amino acid residues existing in the carboxy-terminal region of a gene sequence coding a bile salt activated lipase. The obtained gene is integrated to an EcoRI site of a plasmid pHIL301 and Pichia pastoris GSl15 strain is transformed by the plasmid. The transformant is proliferated at about 30 deg.C and a dissolved oxygen content of 30-50% and cultured in a methanol-containing medium at 15-25 deg.C and a dissolved oxygen content of <20% to effect the induction and expression of bile salt activated lipase in the medium. The recombinant bile salt activated lipase can be expressed in high yield by this process.