| 摘要 |
A method to grow in vitro TIL cell is described, comprising the steps as follows: - a single cell dissolution of a tumourous tissue; - a said cell first incubation in a culture medium with a serum, that allows the lymphocyte growth, comprising interleukin 2 and phytolectin, for an interval from 3 to 5 days at 37 DEG C in order to provide a primary culture; - an isolation of mononucleated cell of said primary culture; and - a second incubation of said monucleated cells in a culture medium, that allows the lymphocytes growth, comprising Interleukin-2, for an interval from 10 and 20 days at 37 DEG C. Preferably, the method is used to grow TIL cells from NSSC. |
