发明名称 |
Methods of detection of mutagens using luminescence gene. |
摘要 |
A method for detecting or quantitating a mutagenic substratance in a sample includes culturing a host microorganism transformed with a recombinant gene comprising an SOS gene and genes expressing luciferase activity and optionally genes expressing an enzyme which catalyzes the production of a substrate for luciferase, positioned downstream of the SOS gene, in a medium to which the sample is added; and measuring a luminescence generated by expression of the gene expressing luciferase activity. The method is sensitive, accurate and non-time consuming; and gene systems used for said method, i.e., a recombinant gene comprising an SOS gene expressed when a DNA is damaged and a gene expressing luciferase activity positioned downstream of the SOS gene, and a host microorganism transformed with said recombinant gene. Preferably the recombinant gene further comprises a gene expressing an enzyme which catalyses the production of a substrate for the luciferase in the down stream of the SOS gene. |
申请公布号 |
EP0649905(A1) |
申请公布日期 |
1995.04.26 |
申请号 |
EP19940307739 |
申请日期 |
1994.10.21 |
申请人 |
KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO |
发明人 |
IMAEDA, TAKAO;HIRAI, MASANA |
分类号 |
C07K14/47;C12N15/53;C12Q1/02;C12Q1/68 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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