| 摘要 |
Homogeneous assays for determining polynucleotide analytes are disclosed. The analyte is detected by a process wherein the binding of a complementary probe to the analyte allows the binding of an intercalating agent or a groove binder. The complementary probe and duplex binder carry different partners of a ligand-to-metal energy transfer system which comprises an energy donor and an energy acceptor. Figure 1 illustrates an embodiment wherein probe DNA is linked to a chelator e.g. EDTA, DTPA, DCTA and the intercalator is linked to a sensitiser. The chelator and sensitiser form a complex around a lanthanide ion e.g. europium, terbium, samarium, dysposium. The system is irradiated with light which can be selectively absorbed by the sensitiser and which can efficiently donate its energy to the lanthanide ion because the metal ion is chelated to the sensitiser. The delayed luminescence of the lanthanide ion marks the binding of the probe to the analyte. Also disclosed are duplex binders linked to (a) sensitisers, (b) chelating agents and (c) energy transfer acceptors. <IMAGE>
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