| 摘要 |
PURPOSE:To provide a new DNA fragment containing a gene coding SecA protein originated from coryneform bacteria and giving a transformed strain containing the gene, capable of producing various kinds of membrane proteins and secretory proteins and utilizable for the production of various kinds of valuable substances. CONSTITUTION:A coryneform bacterium [e.g. Brevibacterium flavum MJ-233 (FERM BP-1497)] is cultured in a medium to the latter term of the logarithmic growth phase, treated successively with buffer solutions containing lysozyme and protease K and subjected to bacteriolysis by adding a surfactant. A DNA is obtained from the lysed liquid by conventional method. A PCR reaction is carried out by using the DNA as a template and using a primer consisting of a part of the SecA gene of e.g. Escherichia coli to obtain the objective new DNA fragment containing a gene coding SecA protein. A transformant capable of producing various kinds of membrane proteins and secretory proteins and utilizable for the production of various kinds of valuable substances can be produced by transforming a host with a recombinant gene containing the fragment bonded to a vector. |
