Menetelmä luonnollisen proteiinimakeuttamisaineen valmistamiseksi

摘要

Thaumatin II or thaumatin I can be obtained through the expression, not of their natural genes, but of artificial, synthetic and substantially optimized genes following specific rules. Preferably, this expression is carried out in filamentous fungi, especially GRAS fungi and particularly the species Penicillium roquefortii, Aspergillus niger and the awamori variant of Aspergillus niger. Preparing substantially optimized artificial genes for filamentous fungi, performed here for the first time in the case of thaumatin, allows for high protein expression, making the process useful for industrial production of this valuable sweetener. Thaumatins may be obtained extracellularly by using a plasmid with a secretion signal, and also intracellularly. The latter method can be used in animal feed without prior separation from the fungal mycelium.