| 发明名称 |
Reverse transcription with Thermus thermophilus polymerase |
| 摘要 |
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, and recombinant Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
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| 申请公布号 |
US5407800(A) |
申请公布日期 |
1995.04.18 |
| 申请号 |
US19930080243 |
申请日期 |
1993.06.17 |
| 申请人 |
HOFFMANN-LA ROCHE INC. |
发明人 |
GELFAND, DAVID H.;MYERS, THOMAS W. |
| 分类号 |
C12N9/12;C12N15/70;C12P19/34;C12Q1/68;C12Q1/70;(IPC1-7):C12Q1/68;C07H5/04;C07H17/00 |
主分类号 |
C12N9/12 |
| 代理机构 |
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代理人 |
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