| 摘要 |
The invention relates to a method of assay for the determination of analytes in samples. In the method, for each analyte to be assayed, monodisperse particles carrying a specific binding partner for that analyte are used to bind the analyte in the sample and a labelled ligand is used to indicate the amount of bound analyte by means of a flow cytometer. For each analyte to be assayed, a pair of different particle types is used, the particles of each of the two particle types of the pair carrying a binding partner having the same specificity but having a different binding affinity for the analyte. The pair of particle types which has reacted with each analyte to be assayed and has become labelled by a labelled ligand is distinguishable by the flow cytometer from each other and from the pairs of particle types which have reacted with other analytes. This makes it possible to avoid the so-called hook-effect. The invention is particularly useful as a method of immunoassy, eg. for the determination of antigens in serum samples.
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