发明名称 |
USE OF CAPILLARY ELECTROPHORESIS FOR QUANTITATING THE CONCENTRATION OF PROTEIN COMPONENTS AND OF THE TOTAL PROTEIN IN FLUIDS |
摘要 |
A method of quantitating proteins in complex samples using capillary electrophoresis can be used both to determine the concentration of a protein in a sample and to determine total protein concentration in the sample. In general, the method of determining the concentration of a marker protein comprises: (1) adding a known quantity of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group consisting of benzoic acid substituted with at least one halogen, producing a detector signal in relation to its concentration, and being capable of electrophoretic separation from the protein; (2) subjecting the sample and the internal standard compound to capillary electrophoresis to separate the protein and the internal standard compound from each other and from other components in the sample; (3) measuring the detector signal produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to internal standard signal; and (4) determining the concentration of the protein in the sample from a standard curve of protein concentration versus the ratio of protein signal to internal standard signal. A typical protein measurable by the method is albumin. Typically, measurements are made at 214 nm and the internal standard compound is 2,4-dichlorobenzoic acid.
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申请公布号 |
CA2149903(A1) |
申请公布日期 |
1995.04.13 |
申请号 |
CA19942149903 |
申请日期 |
1994.09.15 |
申请人 |
BECKMAN INSTRUMENTS, INC. |
发明人 |
WANG, HANN-PING;LIU, CHENG-MING |
分类号 |
G01N33/68;G01N21/33;G01N21/79;G01N27/447;(IPC1-7):G01N27/447;G01N27/26 |
主分类号 |
G01N33/68 |
代理机构 |
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