CHARACTERIZATION OF MYCOPLASMA HYOPNEUMONIAE ADHESINS

摘要

The present invention provides for the identification, purification and characterization of Mycoplasma hyopneumoniae adhesins. The Mycoplasma hyopneumoniae adhesins of the invention were purified using an affinity chromatography procedure which incorporates a novel Mycoplasma hyopneumoniae receptor analogue for the attachment and/or removal of the adhesins from a mycoplasmal preparation. In another aspect of the invention, there is provided an in vitro microtiter plate adherence assay for the characterization of Mycoplasma hyopneumoniae and adhesins thereof. In addition, the effect of passage level on adherence of Mycoplasma hyopneumoniae was evaluated; the selection of high-adherent and low-adherent clones conducted; and monoclonal antibodies against the adhesins were raised and used to study adherence inhibition.