COATED CAPILLARY COLUMNS AND ELECTROPHORETIC SEPARATION METHODS FOR THEIR USE

摘要

Coated capillary electrophoresis columns and methods for their use in electrophoretic separations are disclosed. The coated capillary columns include a length of tubing having an interior surface coated with at least one cross-linked organic multivalent ionic compound capable of ionic interaction with charged chemical functionalities on the tubing interior surface. Exemplary columns are prepared by cross-linking a multi-functional amine compound with a diepoxide, alkylating the cross-linked multi-functional amine compound to form a cross-linked quaternary ammonium compound, and then contacting the interior surface of capillary tubing with the cross-linked quaternary ammonium compound.