摘要 |
<p>A method for preparing rhodamine derivatives of formulae (a) or (b) or a mixture of such compounds, where X is bromo, chloro- or iodoacetamido, maleimido, or amino. These compounds are the 5 and 6 (or 5' and 6') isomers of the amine, haloacetamide and maleimide derivatives of tetramethylrhodamine. The method enables for the first time the production of isomerically pure compounds on a relatively large scale. The products are fluorescent and are useful for the labelling of proteins.</p> |