Modified human granulocyte macrophage-colony stimulating factor gene and expression thereof in yeast.

摘要

Provided herein are: a modified human granulocyte macrophage-colony stimulating factor("hGM-CSF") gene useful for the mass production of hGM-CSF in yeast, an expression vector containing said gene, a yeast cell transformed with said expression vector and a process for producing hGM-CSF by employing the yeast cell. The modified hGM-CSF gene possesses yeast preferred codons and is able to inactivate two N-linked glycosylation recognition sites present in native GM-CSF. <IMAGE>